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Last revised:
September 2009

DSMZ Human and Animal Cell Lines Database

Address DSMZ - Deutsche Sammlung von
Mikroorganismen und Zellkulturen GmbH
Inhoffenstr. 7b
D - 38124 Braunschweig - Germany
Phone +49(0)531 26 16 - 161
Fax +49(0)531 26 16 - 150
Email mutz@dsmz.de
WWW http://www.dsmz.de
Conditions Cell lines are distributed to qualified investigators for use in their own research. Before shipment, the investigator is asked to sign an agreement stating that:

    the cell line will be used for research purposes only
    the cell line shall not be sold or used for commercial purposes
    the cell line shall not be distributed to third parties
    the name of the cell line shall not be changed
    the recipient of the cell line accepts all risks and the conditions of delivery of the DSMZ.

To validate an order a signed Agreement Form has to be sent to us via fax. Without this DSMZ-Order/Agreement Form orders will not be carried out.

Contact Person Herbert Milch (hem@dsmz.de)
Additional Database Information DSMZ_MUTZ


Example of an entry from the DSMZ_MUTZ catalogue.

Accession_number ACC 1
Cell_line_name P-815
Brief_description confirmed as mouse with IEF of LDH, NP, PEP B established from the mastocytoma tumor of a DBA/2 mouse treated with methylcolanthrene; used as target cells for cytotoxic T cell assays; as reported cells exhibit no effector activity in an antibody-dependent cell mediated cytotoxic system
Description mouse mastocytoma established from the mastocytoma tumor of a DBA/2 mouse treated with methylcolanthrene; used as target cells for cytotoxic T cell assays; as reported cells exhibit no effector activity in an antibody-dependent cell mediated cytotoxic system confirmed as mouse with IEF of LDH, NP, PEP B
Depositor Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
Bibliographic_reference Ralph et al., Biochem. Biophys. Res. Commun. 61: 1268-1275 (1974); Ralph et al., Nature 249: 49-51 (1974); Ralph et al., J. Exp. Med. 143: 1528-1553 (1976); Ralph et al., J. Immunol. 119: 950-954 (1977)
Morphology single, round cells in suspension; some cells may be adherent
Culture_conditions 90% RPMI 1640 + 10% FBS maintain at 0.5-5 x 105 cells/ml, cells grow very fast; split 1:5 to 1:10 every 3-4 days; seed out at ca. 1-2 x 106 cells/10 ml at 37C with 5% CO2 maximum cell density at > 1 x 106 cells/ml
Viruses ELISA: reverse transcriptase negative
Properties -
Release_conditions General restrictions
Hazard -
Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule
Doubling_time doubling time of ca. 18-22 hours
Mycoplasma negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Cytogenetics murine near-diploid karyotype; 41(36-43)<2n>; 1-2 centric fusion markers present

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