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ECACC Animal Cell Lines Database Address European Collection of Cell Cultures (ECACC) Salisbury Wiltshire SP4 0JG England Phone +44 (0) 1980 612512 Fax +44 (0) 1980 611315 Email ecacc@hpa.org.uk WWW http://www.ecacc.org.uk/default.asp Conditions All orders subject to ECACC's standard Terms and Conditions HAVE YOU REMEMBERED TO ENCLOSE THE FOLLOWING WITH YOUR ORDER? 1. Please Fax your VAT Exemption Certificate (if eligible) - UK only. 2. Purchase Order number. Or remit your order to: European Collection of Cell Cultures, Salisbury, Wilts, SP4 0JG, UK. Or fax: +44 (0) 1980 611315 We regret that we are unable to accept orders by telephone. Contact Person - Additional Database Information ECACC_CELL Example of an entry from the ECACC_Cell catalogue. Accession_number ECACC 84100401 Cell_line_name MRC-5 SV2 Brief_description Human fetal lung, SV40 transformed Description Human fetal lung, SV40 transformed Depositor Dr R Holliday, NIMR, London, UK Bibliographic_reference J Cell Sci 1983;63:77 Morphology Fibroblast Culture_conditions EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Viruses See Properties Properties Applications: Transformation and virus studies: SV40 Release_conditions No restrictions. Patent: None Specified By Depositor Hazard CZ-II Karyotype Modal No = 60-80, hyperdiploid Comments Derived from the parental cell line MRC-5 by transformation with the SV40 virus. The cells do not senesce. Research_council_deposit Yes Price_code B Top of Page © The CABRI Consortium 1999-2003. This work cannot be reproduced in whole or in part without the express written permission of the CABRI consortium. Home    Description    Search Catalogues    Browse catalogues    Collections    Quality Guidelines Search Web Site    Contacts    FAQ    Site Map    Mirrors Site maintained by the CABRI webmaster. Last revised on December 5, 2003.

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