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Maueler et al., Oncogene, 3: 57 (1988) [PMID: ] CABRI:DSMZ_MUTZ Accession_number ACC 276 Cell_line_name A-2 Brief_description derived from the sweetwater poeciliid fish (xiphophorus xiphidium); origin: Rio Purificacion; strain: Y F1 , embryonal Description fish Poeciliid (Xiphophorus xiphidium) derived from the sweetwater poeciliid fish (xiphophorus xiphidium); origin: Rio Purificacion; strain: Y F1 , embryonal Depositor Prof. M. Schartl, University of Wuerzburg, Wuerzburg, Germany Morphology epitheloid cells growing as monolayer Culture_conditions 80-85% mixture of Ham's F12 + Dulbecco's MEM (at 1:1) + 15-20% FBS split culture 1:3 about 2-3 times per week before cells reach confluency; cells grow loosely adherent and can be detached from the plastic surface by shaking or pipetting; old cultures tend to form foci and lose adherence, but the cells remain attached to each other and are difficult to disperse at 26-28C with 5% CO2 maximal density of 6-10 x 10 6 cells/80 cm 2 Viruses ELISA: reverse transcriptase negative Properties Release_conditions - Hazard - Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 0.5-3.0 x 106 cells/ampoule Doubling_time about 2-3 days Mycoplasma negative in DAPI, microbiological culture, RNA hybridization assays Bibliographic_reference Kuhn et al., In Vitro, 15: 57 (1979) [PMID: 528010 ]

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