Data entry and validation

1. Cell line name

Field	Cell line name
Description	name given to the cell, originally name given by the depositor
Input process	new entry for existing cells:CABRI agreed list of cell names new entry for new cells: as given by depositor existing catalogues: retrieval by SRS should be possible using list of every possible spelling of a name.
Required for	MDS

2. Accession number

Field	Accession number
Description	collections’ own accession number
Input process	automatically given, to avoid duplicates or absence of identifier
Note	a CABRI accession number is given, but only for internal purposes
Required for	MDS

3. Brief description

Field	Brief description
Description	brief listing of : - species - strain - tissue - tumour - pathology - transformed/transfected
Input process	Reference lists: - ‘Dorland’s Illustrated Medical Dictionary’, 27th edition, Saunders -strains are in accordance with the ‘Subsidiary of Harcourt Brace Jovanovich, Publishers’, Academic Press: The Laboratory Rat, The Mouse in Biomedical Research, The Biology of the Laboratory Rabbit -pathologies are in accordance with the ‘On-Line Mendelian Inheritance in Man (OMIM)’,http://www.ncbi.nlm.nih.gov/Omim - CABRI agreed lists:species,tissues,tumours
Required for	MDS

4. Description and other remarks on the cell line

Field	Description and other remarks on the cell line
Description	contain ["verbose" listing] of: - species - strain - tissue - tumour - pathology - transformed/transfected - age - sex - derived from
Input process	Reference lists: - ‘Dorland’s Illustrated Medical Dictionary’, 27th edition, Saunders -strains are in accordance with the ‘Subsidiary of Harcourt Brace Jovanovich, Publishers’, Academic Press: The Laboratory Rat, The Mouse in Biomedical Research, The Biology of the Laboratory Rabbit -pathologies are in accordance with the ‘On-Line Mendelian Inheritance in Man (OMIM)’,http://www.ncbi.nlm.nih.gov/Omim - CABRI agreed lists:species,tissues,tumours
Required for	MDS

5. Depositor name and address

Field	Depositor name and address
Description	depositor name address of the Institution
Input process	CABRI agreed list for country: name based on country code file retrieved from:ftp://ftp.ripe.net/iso3166-countrycodes updated by the RIPE Network Co-ordination Centre, in co-ordination with the ISO 3166 Maintenance Agency at DIN Berlin. Exception for UK, USA, USSR, Netherlands, North Korea
Required for	MDS

6. Reference paper

Field	Reference paper
Description	original paper [if available]
Input process	new entry: JournalTitle Year ; Volume ( issue ): beginning page# - ending page# The title is abbreviated following international standard rules (ISSN). Abbreviations are without dot. Authors and title of the article are not mentioned. existing catalogues: conversion by SRS following CABRI agreed standard This reference can be followed by the Pubmed ID enclosed within square brackets as follows: [PMID: 1234567], where '1234567' is the Pubmed ID of the paper
Required for	MDS

7. Morphology and growth characteristics

Field	Morphology and growth characteristics
Description	Morphology growth characteristics (monolayer, suspension, etc.)
Input process	Reference lists: -morphology: usingCABRI agreed list - ‘Dorland’s Illustrated Medical Dictionary’, 27th edition, Saunders
Required for	MDS

8. Culture conditions

Field	Culture conditions
Description	culture medium subculture routine incubation harvest
Input process	respect order of the items 1. culture medium, 2. subculture routine 3. incubation, 4. Harvest
Required for	MDS

9. Viruses

Field	Viruses
Description	absence/presence type of virus
Input process	In case of absence of data related toViruses, input the following sentence:"There is no evidence for the presence of infectious viruses". Reference lists: -CABRI agreed list - ‘Review of Medical Microbiology’, seventeenth ed., Appleton & Lange; - the WWW biological site ‘All the virology on the WWW’, (http://www.tulane.edu/~dmsander/garryfavweb.html)
Required for	MDS

10. Properties

Field	Properties
Description	Immunology receptors susceptibility to viruses
Input process	Free text
Requred for	MDS

11. Passage

Field	Passage
Description	passage no
Input process	when unknown, put ‘unknown’
Required for	RDS

12. Release conditions/Restrictions

Field	Release conditions/Restrictions
Description	release conditions/restrictions patents
Input process	release conditions/restrictions: enter "no", or a text field describing the appropriate restrictions: hazard group, import/export regulations, .. The text may contain codes which refer to text files explaining the restrictions in more detail. patents: EPO standard rules
Required for	MDS

13. Hazard

Field	Hazard
Description	hazard classification of the cell line [e.g. class II containment] EU related hazard information rules country-specific related hazard information rules
Input process	- classifications available on the CABRI Web site: see Guidelines for Quality Management - ACDP category list:http://www.doh.gov.uk/bioinfo.htm
Required for	MDS

14. Species validation tests

Field	Species validation tests
Description	Should contain tests and results of tests made to confirm species of cell lines
Input process	CABRI agreed list
Required for	RDS