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LABORATORY PROCEDURES FOR ANIMAL & HUMAN CELL LINES

Appendix

REFERENCE NO: AHC/1998/3/1.2 Appendix 4


TITLE: INACTIVATION OF FOETAL BOVINE SERUM


INTRODUCTION

Inactivated foetal bovine serum, FBS, is used for making up media.

PROCEDURE

  1. Take bottle of FBS and warm to 37 ± 1°C.
  2. Heat water bath to 56 ± 1°C.
  3. Place warmed bottle of FBS in water bath at 56 ± 1°C for 30 minutes.
  4. Remove and allow bottle to cool.

 

Spray bottle with 10% alcohol before taking to tissue culture cabinet.


Guidelines prepared for CABRI by CERDIC, DSMZ, ECACC, INRC, November 1998
Page layout by CERDIC
Copyright CABRI, 1998


© The CABRI Consortium 1999-2003. This work cannot be reproduced in whole or in part without the express written permission of the CABRI consortium.

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