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CABRI Guidelines

LABORATORY PROCEDURES FOR MICROORGANISMS

Part 1: Introduction and general documents

Procedures

Appendixes

Part 2: Request and handling of deposits

Flow diagram

Procedures

Appendixes

Part 3: Maintaining deposits

Flow diagrams

Procedures

Appendixes

Part 4: Delivery of material to customers

Flow diagram

Procedures

Appendixes

 


 Part 1: Introduction and general documents - Procedures -

Reference Number

Procedure Title

M/1999/1.00

Introduction & general documents

M/1999/1.01

Establishment and operation of culture collections

M/1999/1.02

International laws and regulations concerning the collection work

M/1999/1.03

European laws and regulations concerning the collection work

M/1998/1.04

National laws and regulations concerning the collection work

M/1998/1.05

Security, quality and transfer restriction codes

M/1998/1.06

Introduction to safety regulations

M/1998/1.07

Issue, control and updating of laboratory procedures

M/1998/1.08

Traceability of data


Part 1: Introduction and general documents -
Appendixes -

Reference Number

Appendixes

M/1999/1.00 Appendix 1

WFCC Guidelines for the Establishment and Operation of Collections of Cultures of Microorganisms

M/1998/1.05 Appendix 1

Potentially pathogenic materials

M/1998/1.05 Appendix 2

Potentially hallucenogenic materials

M/1998/1.05 Appendix 3

Potential toxin producers

M/1998/1.05 Appendix 4

Transfer agreement

M/1998/1.06 Appendix 1

Safe handling of liquid nitrogen

M/1998/1.06 Appendix 2

First aid: Laboratory acquired infections

M/1998/1.06 Appendix 3

Good Laboratory Practice

M/1998/1.06 Appendix 4

Unpacking of cultures received for deposit

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Part 2: Guidelines for requesting and handling new deposits - Procedures -

Reference Number

Procedure Title

M/1998/2.00

Accession of Microorganisms

M/1998/2.01

Accession form

M/1998/2.02

Recording collection data

M/1998/2.03

Transfer of cultures

M/1998/2.04

Storage of original culture material

M/1998/2.05

(Preliminary) quality check

M/1998/2.06

Acknowledgement of receipt of a deposit

M/1998/2.07

Depositor check

M/1998/2.08

Release of cultures for dispatch

M/1998/2.09

Transfer of strain data to database

 

Part 2: Guidelines for requesting and handling new deposits - Appendixes -

Reference Number

Appendixes

M/1998/2.00 Appendix 2.1

Accession criteria BCCM

M/1998/2.00 Appendix 2.2

Accession criteria CBS

M/1998/2.00 Appendix 2.3

Accession criteria DSMZ

M/1998/2.00 Appendix 3.1

Standard letter: Request of strain

M/1998/2.00 Appendix 4.1

Standard letter: Rejection of strain

M/1998/2.01 Appendix 1

Accession form BCCM-LMG

M/1998/2.01 Appendix 2

Accession form BCCM-IHEM

M/1998/2.01 Appendix 3

Accession form BCCM-MUCL

M/1998/2.01 Appendix 4

Accession form CBS

M/1998/2.01 Appendix 5

Accession form DSMZ

M/1998/2.02 Appendix 1

Day Book, example DSMZ

M/1998/2.02 Appendix 2

Accession Book

M/1998/2.03 Appendix 1

Preparation and storage of media

M/1998/2.03 Appendix 2

Preparation of prereduced media

M/1998/2.05 Appendix 1

Selected tests for quality control

M/1998/2.05 Appendix 2

Procedure for preliminary quality check

M/1998/2.06 Appendix 1

Standard letter

M/1998/2.07 Appendix 1

Standard letter

M/1998/2.07 Appendix 2

Depositor's reply form

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Part 3: Guidelines for maintaining deposits - Flow diagrams -

Reference Number

Flow diagrams

M/1998/3.00 Appendix 1

Maintenance and preservation

M/1998/3.00 Appendix 2

Preservation methods

M/1998/3.00 Appendix 5.07.1

Ultra-low cryopreservation

M/1998/3.00 Appendix 5.08.1

Freeze-drying process

M/1998/3.00 Appendix 5.10.1

Centrifugal freeze-drying

M/1998/3.00 Appendix 5.11.1

Vacuum-drying

M/1998/3.00 Appendix 5.13.1

Freeze drying of fungi

M/1998/3.02 Appendix 1

Monitoring viability and stock numbers

M/1998/3.02 Appendix 2

Stock control, frozen cultures

M/1998/3.02 Appendix 3

Stock control, (freeze)-dried cultures

 

Part 3: Guidelines for maintaining deposits - Procedures -

Reference Number

Procedure Title

M/1998/3.00

Maintenance and Preservation of Microorganisms

M/1998/3.01

Storage of preserved microorganisms

M/1998/3.02

Stock control and periodic quality control

 

Part 3: Guidelines for maintaining deposits - Appendixes -

Reference Number

Appendixes

M/1998/3.00 Appendix 3

Protective suspension media for freezing or (freeze)-drying

M/1998/3.00 Appendix 4

Protocol form: Protective suspension media

M/1998/3.00 Appendix 5.00

Survey of detailed preservation methods

M/1998/3.00 Appendix 5.01

Preservation of bacteria by freezing and low temperature storage in glass capillary tubes

M/1998/3.00 Appendix 5.01.1

Protocol form: Preservation of bacteria in LN2

M/1998/3.00 Appendix 5.01.2

Protocol form: Quantity of frozen stock cultures

M/1998/3.00 Appendix 5.01.3

Protocol form: Position of cultures in LN2

M/1998/3.00 Appendix 5.02

Preservation of bacteria by freezing and low temperature storage in polypropylene straws

M/1998/3.00 Appendix 5.03

Preservation of sporoactinomyces by freezing and low temperature storage in PVC straws

M/1998/3.00 Appendix 5.04

Preservation of bacteria above liquid nitrogen in cryotubes

M/1998/3.00 Appendix 5.05

Preservation of bacteria by freezing and low temperature storage below -70C on glass beads

M/1998/3.00 Appendix 5.05.1

Protocol Form: Preservation of bacteria by freezing and storage below -70C on glass beads

M/1998/3.00 Appendix 5.06

Preservation of bacteria on ceramic beads below -70C

M/1998/3.00 Appendix 5.07

Cryopreservation of fungi

M/1998/3.00 Appendix 5.07.2

Comments: Ultra-low cryopreservation

M/1998/3.00 Appendix 5.08

Preservation of bacteria by freeze-drying (1)

M/1998/3.00 Appendix 5.08.2

Protocol form: (Freeze)-drying of bacteria

M/1998/3.00 Appendix 5.09

Preservation of bacteria by freeze-drying (2)

M/1998/3.00 Appendix 5.10

Preservation of bacteria by centrifugal freeze-drying

M/1998/3.00 Appendix 5.11

Preservation of bacteria by vacuum-drying

M/1998/3.00 Appendix 5.11.2

Protocol form: Predried skim milk batches

M/1998/3.00 Appendix 5.12

Preservation of bacteria by liquid-drying (L-drying)

M/1998/3.00 Appendix 5.13

Preservation of fungi by freeze-drying

M/1998/3.00 Appendix 5.13.2

Comments: Freeze drying of fungi

M/1998/3.00 Appendix 5.14

Opening of ampoules and rehydration of (freeze)-dried cultures

M/1998/3.02 Appendix 4

Procedure for estimating survival rates: bacteria, yeasts

M/1998/3.02 Appendix 5

Procedures estimation of survival after preservation: fungi

M/1998/3.02 Appendix 6

Viability check book

M/1998/3.02 Appendix 7

Preservation book

M/1998/3.02 Appendix 8

Note for entering cultures into the stock/delivery datafile

 

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Part 4: Guidelines for delivering material to customers - Procedures -

Reference Number

Procedure Title

M/1998/4.00

Ordering and dispatch of cultures

M/1998/4.01

Procedure for dealing with customers complaints

Part 4: Guidelines for delivering material to customers - Appendixes -

Reference Number

Appendixes

M/1998/4.01 Appendix 1

Form: Complaints

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Guidelines prepared for CABRI by DSMZ, CBS and BCCM, 17 May 1998; updated August 1999
Page layout by CERDIC
Copyright CABRI, 1999

© The CABRI Consortium 1999 - 2023
This work cannot be reproduced in whole or in part without the express written permission of the CABRI consortium.
Site maintained by Paolo Romano. Last revised on February 2023.