CABRI Guidelines

LABORATORY PROCEDURES FOR PLANT CELL VIRUSES

Part 1: Introduction and general documents

Procedures

Part 2: Request and handling new deposit

Flow diagrams

Part 3: Maintaining deposits

Procedures

Part 4: Delivery of material to the customer

Flow diagram

Part 1: Introduction and general documents - Procedures -

Reference Number	Procedure Title
PVC/1999/1.01	International laws and regulations concerning the collection work
PVC/1998/1.02	European laws and regulations concerning the collection work
PVC/1998/1.03	National laws and regulations concerning the collection work in Germany
PVC/1998/1.04	Introduction to safety regulations
PVC/1998/1.05	Issue, control, and updating of laboratory procedures

Part 1: Introduction and general documents - Appendixes -

Reference Number	Appendixes
PVC/1998/1.04 Appendix 1	First aid after accidental exposure to infectious microorganisms
PVC/1998/1.04 Appendix 2	Good microbiological practice
PVC/1998/1.04 Appendix 3	Safe handling of liquid nitrogen (LN2)

Part 2: Guidelines for requesting and handling new deposits - Flow diagrams -

Reference number	Flow diagrams
PVC/1998/2.00 Appendix 1	Accession of virus infected plant material
PVC/1998/2.02 Appendix 1	Procedure for preliminary quality control
PVC/1998/2.03 Appendix 1	Authenticity Screen
PVC/1998/2.04 Appendix 1	Full authentication

Part 2: Guidelines for requesting and handling new deposits - Procedures -

Reference number	Procedure Title
PVC/1998/2.00	Accession of virus infected plant material
PVC/1998/2.01	Storage of the original virus infected plant materials
PVC/1998/2.02	Quality Control Check
PVC/1998/2.03	Authenticity Screen
PVC/1998/2.04	Full authentication

Part 2: Guidelines for requesting and handling new deposits - Appendixes -

Reference number	Appendixes
PVC/1998/2.00 Appendix 2	Standard letter: Request of virus infected plant material
PVC/1998/2.00 Appendix 3	Standard letter: Reply when virus infected plant material is offered
PVC/1998/2.00 Appendix 4	Standard letter: Rejection of virus infected plant material
PVC/1998/2.00 Appendix 5	Accession form
PVC/1998/2.00 Appendix 6	Database form
PVC/1998/2.00 Appendix 7	Standard letter: Acknowledgement of receipt
PVC/1998/2.02 Appendix 2	Mechanical transmission
PVC/1998/2.02 Appendix 3	Grafting of material to healthy host plants
PVC/1998/2.02 Appendix 4	Transmission by insect vectors
PVC/1998/2.02 Appendix 5	Electron microscopy - Leaf dip assay
PVC/1998/2.03 Appendix 2	Immuno Electron Microscopy
PVC/1998/2.03 Appendix 3	Clean up by mechanical transmission to differential host
PVC/1998/2.03 Appendix 4	Clean up by selective vector transmission
PVC/1998/2.04 Appendix 2	Serotyping by ELISA

Part 3: Guidelines for maintaining deposits - Procedures -

Reference number	Procedure Title
PVC/1998/3.00	Preservation of virus infected plant materials, maintenance and stock control
PVC/1998/3.01	Preservation of virus infected plant material by freezing and low temperature storage in liquid nitrogen
PVC/1998/3.02	Preservation of virus infected plant material by freeze drying and low temperature storage
PVC/1998/3.03	Virus maintenance in situ

Part 4: Guidelines for delivering material to customers - Procedures -

Reference number	Procedure Title
PVC/1998/4.00	Ordering and dispatch of cultures
PVC/1998/4.01	Procedure for dealing with customers complaints

Guidelines prepared for CABRI by DSMZ, 3 Feb. 1998; updated August 1999
Page Layout by CERDIC
Copyright CABRI, 1998

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