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LABORATORY PROCEDURES FOR PLANT CELL VIRUSES

REFERENCE NO.: PVC/1998/2.03


TITLE: AUTHENTICITY SCREEN


INTRODUCTION

After successful transmission and establishment of virus infections in respective herbaceous host plants, symptoms in indicator plants are evaluated and the material is subjected to electron microscopical examinations in leaf dip assays and in immuno electron microscopy. This is especially indicated with material of which no prior knowledge exists, e.g. which is field sampled material. In the authenticity screen, a combination of methods is applied to ensure purity of the virus isolate and/or to clean up material which is either a mixed infection of different viruses contributing to a disease syndrom or, a mixed infection with a contaminating virus.

The techniques listed in PVC/1998/2.03/ Appendix 1 are tools which are used alone or in combination to respond to the situation as is presented by the biological characteristics of the virus present. The authentication process is closely monitored by EM and IEM. Due to the laborious and cumbersome process of cleaning up mixed infections, when appropriate, the depositor is contacted at a very early stage, often after a mixed virus infection has been diagnosed.

 

LIST OF GLASSHOUSE AND LABORATORY PROCEDURES

PVC/1998/2.02 Appendix 2 Mechanical transmission
PVC/1998/2.02 Appendix 3 Grafting of material to healthy host plants
PVC/1998/2.02 Appendix 4 Transmission of virus by insect vectors
PVC/1998/2.02 Appendix 5 Electron microscopy - Leaf dip assay

PVC/1998/2.03 Appendix 2 Immuno electron microscopy
PVC/1998/2.03 Appendix 3 Clean up by mechanical transmission to differential host
PVC/1998/2.03 Appendix 4 Clean up by selective vector transmission


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