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LABORATORY PROCEDURES FOR MICROORGANISMS

Flow diagram

M/1998/3.00 Appendix 5.07.1


CRYOPRESERVATION OF FUNGI

  

Select procedure

¯

Submerge inocula in cryoprotectant in cryovial

¯

Label cryovial

¯

Cool with selected cooling rate (general 1°C/min.) to 45° C

store below -130 °C

¯

Check viability and quality after 16 h and 5 year (specialist)

(Revival 5 min. waterbath 30°C; Oomycota and sensitive organisms at 25° C)


Guidelines prepared for CABRI by DSMZ, CBS and BCCM, 17 May 1998
Page layout by CERDIC
Copyright CABRI, 1998

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Site maintained by Paolo Romano. Last revised on February 2023.