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LABORATORY PROCEDURES FOR ANIMAL & HUMAN CELL LINES

Quality controls at DSMZ

 

Identification of cell line		Mycoplasma testing
Reference Number	Procedure Title	Reference Number	Procedure Title
AHC/1998/3/3.1/1.1	Cytogenetics (chromosome analysis)	AHC/1998/3/3.2/1.1	Direct DNA fluorescence DAPI staining
AHC/1998/3/3.1/1.2	Isoenzyme analysis	AHC/1998/3/3.2/1.2	Broth-agar microbiogical colony assay
AHC/1998/3/3.1/1.3	DNA fingerprinting	AHC/1998/3/3.2/1.3	RNA hybridization in solution
AHC/1998/3/3.1/1.4	Immunophenotyping	AHC/1998/3/3.2/1.4	PCR
		AHC/1998/3/3.2/1.5	Mycoplasma eradication

 

Bacteria and fungi testing		Virus testing
Reference Number	Procedure Title	Reference Number	Procedure Title
AHC/1998/3/3.3/1.1	Bacteria and Fungi detection by culture	AHC/1998/3/3.4/1.1	PCR analysis for HIV
AHC/1998/3/3.3/1.2	Bacteria and Fungi eradication	AHC/1998/3/3.4/1.2	PCR analysis for HTLV 1,2
		AHC/1998/3/3.4/1.3	PCR analysis for HBV, HCV, HHV-8
		AHC/1998/3/3.4/1.4	PCR analysis for EBV
		AHC/1998/3/3.4/1.5	Retrovirus detection by ELISA