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LABORATORY PROCEDURES FOR PLANT CELL LINES

REFERENCE NO.: PC/1998/2/1


TITLE: GUIDELINES FOR REQUESTING, PROCESSING, CATALOGUING, MAINTENANCE, AND DELIVERY OF PLANT CELL LINES


INTRODUCTION

The public collection of plant cell lines is continually expanded by the process of accessioning new plant cell lines as far as possible dependent upon available resources. This is usually in response to the application of a depositor or by request of the collection. The plant cell lines pass a preliminary quality check and a further final quality check and authentication before they are included in the public collection and further maintained either by continuous subculturing or long term preservation. Routine quality checks are performed to assure the quality standard of the collection. On request of customers, plant cell lines are recovered and delivered. This document describes the procedures for these purposes. PC/1998/2.1 contains a flow chart showing the general procedures for accessioning of new plant cell lines including preliminary quality check, final quality check and authentication.

PROCEDURE

1. Request and receipt of plant cell lines and preliminary quality control (PC/1998/2/2 - PC/1998/2/3.5).

Request and receipt of plant cell lines: Details are described in laboratory documents PC/1998/2/2 - PC/1998/2/2.4.

Preliminary quality control: Details are described in laboratory documents PC/1998/2/3 - PC/1998/2/3.5.

2. Quality control and acceptance of plant cell lines (PC/1998/3/1 - PC/1998/3/2).

Living cultures: Details are described in laboratory documents PC/1998/3/1- PC/1998/3/1.6.

Frozen culture: Details are described in laboratory documents PC/1998/3/2.

3. Routine maintenance and storage of cell lines (PC/1998/3/3 - PC/1998/3/4.2)

Living cultures: Details are described in laboratory documents PC/1998/3/3 - PC/1998/3/3.3.

Frozen cell lines: Details are described in laboratory documents PC/1998/3/4 - PC/97/3/4.2.

4. Delivery of cell lines (PC/1998/4/1).

Delivery of cell lines: Details are described in laboratory documents PC/1998/4/1


Guidelines prepared for CABRI by DSMZ, 20 Jan. 1998
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Copyright CABRI, 1998

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