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LABORATORY PROCEDURES FOR PLANT CELL LINES

Appendix

PC/1998/2/2.2 Appendix 1


TITLE: FORM FOR THE ACCESSION OF PLANT CELL CULTURES


1. PASSPORT DATA

 1.1. CELL CULTURE NAME

 Botanical Name of Initial Plant:

 

Strain Designation:

  

1.2. DEPOSITOR

 Name of Depositor:

 

Institute:

 

Department:

 

Address:

 

 

 

 

Phone number: Fax Number:

Email:

 1.3. TAXONOMIC NAME OF INITIAL PLANT

 Genus name:

Author:

Species name:

Author:

Subspecies name:

Author:

Variety name:

Author:

Cultivar name:

Author:

other designation

Author:

Synonymous names:

(please use additional page if there is more than one synonymous name)

 

1.4. CELL CULTURE HISTORY:

Former institutes and periods of maintenance:

 

 

 Origin of initial plant material:

 

 

 

Reference (Seed or Herbarium Number):

  

 

1.5. CELL CULTURE INITIATION

 Material for culture initiation:

 

 

Method for surface sterilization of initial plant material:

 

  

 

In case of seeds: conditions for germination:

 

 

Cell culture initiator: Date of sterilization:

 

Date of first callus development: Date of culture establishment

 

Medium of callus initiation:

 

 

Method for the initiation of suspension culture:

 

 

 

Applied selection procedure for culture initiation or maintenance:

 

 


2. DATA FOR CULTURE MAINTENANCE

 2.1. GROWTH CONDITIONS

 Growth temperature:

 

Light conditions:

 

Rel. humidity:

 

Other requirements:

 

 

Growth medium:

 

Composition of medium (use additional sheet if necessary):

  

  

 

pH of medium and pH adjustment:

 

Sterilization temperature: Sterilization time:

Filter type for sterile filtration:

 

 

 

2.2. CULTURE HANDLING

 Growth vessel:

 

Amount of Medium:

 

Transfer Period:

 

Transfer method (incl. amount of inoculum in case of suspension cultures):

 

 

 Shaking (rpm):

 

Selection method applied for cell culture maintenance:

 

  

 

Method for Growth Measurement:

 

 

Method for Viability Measurement:

 

Remarks (any remarkable procedure for the medium preparation like: preparation of special stock solutions etc. or for the handling of cultures):

 


3. MORPHOLOGICAL CHARACTERISTICS

 Growth (0 = very bad, 1 = bad, 2 = good, 3 = very good):

 Consistency (from -2 = very soft to +2 = very rigid):

 Colour :

 Morphology:

 Differentiated structures (embryos, shoots, roots):

 


4. SPECIAL TRAITS OF THE CULTURE

(give any metabolite or physiological capacity of this specific culture)

 

 

 

 

 


5. BRIEF DESCRIPTION OF A CRYOPRESERRVATION METHOD

(give only methods that have been successfully applied to this specific culture)

 

 

 

 

 


6. SAFETY AND REGULATORY CLASSIFICATION

Is the culture free of fungi: ( ) yes ( ) no ( ) not tested

bacteria: ( ) yes ( ) no ( ) not tested

viruses: ( ) yes ( ) no ( ) not tested

Which safety classification does the culture belong to (genetically transformed cultures may not be maintained at the moment):

 L1: ( ) L2: ( )

S1: ( ) S2: ( )

 

Restrictions for the delivery of cell cultures:

 

The culture may be delivered without any restrictions: ( )

The culture may be delivered for research purposes only: ( )

 

Date: Signature:

 

 


7. REFERENCES

 

 

 

 


8. LIST OF ATTACHEMENTS

 

 

 

 

 


Guidelines prepared for CABRI by DSMZ, 20 Jan. 1998
Page Layout by CERDIC
Copyright CABRI, 1998 

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This work cannot be reproduced in whole or in part without the express written permission of the CABRI consortium.
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