LABORATORY PROCEDURES FOR PLANT CELL LINES
PC/1998/2/3.3 Appendix 1
Laboratory procedure for bacterial test agar
Tests for bacterial contamination are performed on CASO-Agar. The test agar is prepared as follows:
1. The following components are mixed in a 1 l Schott laboratory flask
CASO-Agar (Merck) 20.0 g
Yeast extract 1.5 g
Glucose 1.25 g
Distilled water 500.0 ml
2. Adjust to pH 7.0
3. Autoclave for 30 minutes at 1.2 bar and 120 °C
4. Pour into petri dishes (60 - 90 mm in diameter with ventilation) under sterile conditions and allow to solidify.
5. Wrap into a plastic bag and store at 4°C.
Guidelines prepared for CABRI by DSMZ, 20 Jan. 1998
© The CABRI Consortium 1999-2013.