LABORATORY PROCEDURES FOR PLANT CELL LINES
REFERENCE NO.: PC/1998/3/1.5
TITLE: PREPARATION HPLC SOLVENTS FOR STANDARD ANALYSIS
The necessary solvents for the standard HPLC procedure have to be prepared as follows.
1. Fill the desired volume of water or methanol in a measuring cylinder
2. Add the corresponding volume of phosphoric acid (0.01 % = 100 ml/l)
3. Pass the solvent through a sterile filter (Millipore GVHP 04700, pore size 0.22 mm or equivalent type) into a freshly cleaned glass bottle.
3.1. If necessary install the filter disk into the filtration device and wet the filter with a small volume of methanol.
3.2. Place the filtration device in the hood and close the safety glass plate.
3.3. Force the solvent through the filter by a vacuum pump.
4. Use the glass bottle directly as reservoir for the HPLC device.
Guidelines prepared for CABRI by DSMZ, 20 Jan. 1998
© The CABRI Consortium 1999-2013.